Legionella and non-Legionella bacteria in a biological treatment plant

Forfatter
Fykse, Else-Marie
Aarskaug, Tone
Thrane, Ingjerd
Blatny, Janet Martha
Publisert
2013
Emneord
Legionella
Bakterier
Permalenke
https://ffi-publikasjoner.archive.knowledgearc.net/handle/20.500.12242/477
DOI
10.1139/cjm-2012-0166
Samling
Articles
Description
Fykse, Else-Marie; Aarskaug, Tone; Thrane, Ingjerd; Blatny, Janet Martha. Legionella and non-Legionella bacteria in a biological treatment plant. Canadian journal of microbiology (Print) 2013 ;Volum 59.(2) s. 102-109
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Sammendrag
Legionella pneumophila were previously identified in the aeration ponds (up to 1010 CFU/L) of a biological wastewater treatment plant at Borregaard Ind. Ltd., Sarpsborg, Norway, and in air samples (up to 3300 CFU/m3) collected above the aeration ponds. After 3 outbreaks of Legionnaires’ disease reported in this area in 2005 and 2008, the aeration ponds of the plant were shut down by the Norwegian authorities in September 2008. The aim of the present work was to analyze the Legionella and non-Legionella bacterial communities in the aeration ponds before and during the shutdown process and to identify potential human pathogens. The non-Legionella bacterial community was investigated in selected samples during the shutdown process by 16S rDNA sequencing of clone libraries (400 clones) and growth analysis. The concentration of L. pneumophila and Pseudomonas spp. DNA were monitored by quantitative PCR. Results showed a decrease in the concentration of L. pneumophila and Pseudomonas spp. during the shutdown. This was accompanied by a significant change in the composition of the bacterial community in the aeration ponds. This study demonstrated that several advanced analytical methods are necessary to characterize the bacterial population in complex environments, such as the industrial aeration ponds.
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