Real-time PCR and NASBA for rapid and sensitive detection of Vibrio cholerae in ballast water

Author
Fykse, Else-Marie
Nilsen, Trine
Nielsen, Agnete Dessen
Tryland, Ingun
Delacroix, Stephanie
Blatny, Janet Martha
Date Issued
2012
Keywords
Ballast vann
Bakterier
Deteksjon
Permalink
http://hdl.handle.net/20.500.12242/769
https://ffi-publikasjoner.archive.knowledgearc.net/handle/20.500.12242/769
DOI
10.1016/j.marpolbul.2011.12.007
Collection
Articles
Description
Fykse, Else-Marie; Nilsen, Trine; Nielsen, Agnete Dessen; Tryland, Ingun; Delacroix, Stephanie; Blatny, Janet Martha. Real-time PCR and NASBA for rapid and sensitive detection of Vibrio cholerae in ballast water. Marine Pollution Bulletin 2012 ;Volum 64.(2) s. 200-206
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Abstract
Transport of ballast water is one major factor in the transmission of aquatic organisms, including pathogenic bacteria. The IMO-guidelines of the Convention for the Control and Management of Ships’ Ballast Water and Sediments, states that ships are to discharge <1 CFU per 100 ml ballast water of toxigenic Vibrio cholerae, emphasizing the need to establish test methods. To our knowledge, there are no methods sensitive and rapid enough available for cholera surveillance of ballast water. In this study real-time PCR and NASBA methods have been evaluated to specifically detect 1 CFU/100 ml of V. cholerae in ballast water. Ballast water samples spiked with V. cholerae cells were filtered and enriched in alkaline peptone water before PCR or NASBA detection. The entire method, including sample preparation and analysis was performed within 7 h, and has the potential to be used for analysis of ballast water for inspection and enforcement control.
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