Multichannel open tubular enzyme reactor online coupled with mass spectrometry for detecting ricin

Author
Brandtzaeg, Ole Kristian
Røen, Bent Tore
Enger, Siri
Lundanes, Elsa
Wilson, Steven Ray Haakon
Date Issued
2017
Keywords
Ricin
Deteksjon
Permalink
http://hdl.handle.net/20.500.12242/830
https://publications.ffi.no/123456789/830
DOI
10.1021/acs.analchem.7b02590
Collection
Articles
Description
Brandtzaeg, Ole Kristian; Røen, Bent Tore; Enger, Siri; Lundanes, Elsa; Wilson, Steven Ray Haakon. Multichannel open tubular enzyme reactor online coupled with mass spectrometry for detecting ricin. Analytical Chemistry 2017 ;Volum 89.(17) s. 8667-8673
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Abstract
For counterterrorism purposes, a selective nano liquid chromatography–mass spectrometry (nanoLC-MS) platform was developed for detecting the highly lethal protein ricin from castor bean extract. Manual sample preparation steps were omitted by implementing a trypsin/Lys-C enzyme-immobilized multichannel reactor (MCR) consisting of 126 channels (8 μm inner diameter in all channels) that performed online digestion of proteins (5 min reaction time, instead of 4–16 h in previous in-solution methods). Reduction and alkylation steps were not required. The MCR allowed identification of ricin by signature peptides in all targeted mode injections performed, with a complete absence of carry-over in blank injections. The MCRs (interior volume ≈ 1 μL) have very low backpressure, allowing for trivial online coupling with commercial nanoLC-MS systems. The open tubular nature of the MCRs allowed for repeatable within/between-reactor preparation and performance.
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