Genetic distribution of 295 Bacillus cereus group members based on adk-screening in combination with MLST (Multilocus Sequence Typing) used for validating a primer targeting a chromosomal locus in B. anthracis

Author
Olsen, Jaran S.
Skogan, Gunnar
Fykse, Else Marie
Rawlinson, Elizabeth L. A.
Tomaso, Herbert
Granum, Per Einar
Blatny, Janet
Date Issued
2007
Permalink
http://hdl.handle.net/20.500.12242/671
https://publications.ffi.no/123456789/671
DOI
10.1016/j.mimet.2007.10.001
Collection
Articles
Description
Olsen, Jaran S.; Skogan, Gunnar; Fykse, Else Marie; Rawlinson, Elizabeth L. A.; Tomaso, Herbert; Granum, Per Einar; Blatny, Janet. Genetic distribution of 295 Bacillus cereus group members based on adk-screening in combination with MLST (Multilocus Sequence Typing) used for validating a primer targeting a chromosomal locus in B. anthracis. Journal of Microbiological Methods 2007 ;Volum 71.(3) s. 265-274
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Abstract
The genetic distribution of 295 Bacillus cereus group members has been investigated by using a modified Multilocus Sequence Typing method (MLST). By comparing the nucleic acid sequence of the adk gene fragment, isolates of B. cereus group members most related to B. anthracis may be easily identified. The genetic distribution, with focus on the B. anthracis close neighbours, was used to evaluate a new primer set for specific identification of B. anthracis. This primer set, BA5510-1/2, targeted the putative B. anthracis specific gene BA5510. Real-time PCR using BA5510-1/2 amplified the target fragment from all B. anthracis strains tested and only two (of 289) non-B. anthracis strains analysed. This is one of the most thoroughly validated chromosomal B. anthracis markers for real-time PCR identification, in which the screened collection contained several very closely related B. anthracis strains.
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